Journal: Cell Host & Microbe

Article Title: SARS-CoV-2 spike L452R variant evades cellular immunity and increases infectivity

doi: 10.1016/j.chom.2021.06.006

Figure Lengend Snippet:

Article Snippet: CF640R succinimidyl ester , Biotium , Cat# 92108.

Techniques: Virus, Recombinant, Modification, Expressing, Reverse Transcription, Transfection, Luciferase, Mutagenesis, Gel Extraction, Cloning, Plasmid Preparation, Quantitative RT-PCR, Competitive Binding Assay, Sequencing, Random Hexamer, Software, Pore Size, Membrane

Journal: bioRxiv

Article Title: Multi-modal Single-molecule Imaging with Continuously Controlled Spectral-resolution (CoCoS) Microscopy

doi: 10.1101/2020.10.13.330910

Figure Lengend Snippet: Resolving the shades of red dyes. a) schematic illustration explaining the procedure to obtain spectral information with CoCoS imaging. First, a localization image is captured using RPA of 180°, then the prisms are rotated to the optimal RPA for the spectral resolution of the imaged dyes (here 120°) and a second image is captured. An intensity profile of a molecule in the spectral image, with respect to the molecule’s coordinates in the localization image, contains the emission spectrum of the molecule. b) Three different red fluorophores were used to label 100 nm silica beads. The beads were mixed and imaged according to the procedure illustrated in a, shown in the false colored FOV image on the left. The localization image presented in white is overlaid on the spectral image in red, showing the dispersed molecular spectra. On the right, the localization image is shown after classification of the dyes according to their spectra using cross-correlation of the individual spectrum with average dye spectra (see methods). Blue, yellow and red refer to CF640R, AF647 and Cy 5.5 respectively. Colored dashed squares mark the molecules presented in panel c. Scale bars are 30 µm. c) Normalized spectral intensity profile of three selected molecules in panel b. Cropped images of the molecules after alignment and Gaussian filtering of the spectral image is shown at the bottom. Dashed lines indicate the localization coordinate. d) An overlay of all individual spectral profiles extracted from the FOV. Mean cross-correlation scores used for the classification of the spectra are shown in the legend. Vertical lines show the theoretical emission maxima of the corresponding dyes’ spectra, showing that molecular spectra with maxima differing by only ~6nm are resolvable with CoCoS.

Article Snippet: For the far-red beads experiment the same procedure was followed mixing a single 75 µM DBCO/BCN activated dye (AF647-DBCO, Cy5.5-DBCO, Jena Bioscience, Germany; CF640R-BCN, Biotium, USA) with the azide-covered nano-beads to create three different stock solutions.

Techniques: Imaging

Journal: iScience

Article Title: Complex sphingolipid profiling and identification of an inositol-phosphorylceramide synthase in Dictyostelium discoideum

doi: 10.1016/j.isci.2024.110609

Figure Lengend Snippet:

Article Snippet: CF640R Goat Anti-Guinea Pig IgG (H + L), Highly Cross-Adsorbed , Biotium , BOT-20494.

Techniques: Virus, Recombinant, Bicinchoninic Acid Protein Assay, Software, Imaging